Electrophoresis of Bacterial Variants1

Electrophoresis of bacterial variants.

Zone electrophoresis of enzymes in bacterial taxonomy.

The electrophoretic mobilities in starch gels have been determined for eight enzymes in extracts of representative cultures of members of the family Enterobacteriaceae. These values were compared with each other and with those obtained from certain bacteria not in this family. The migrations of the eight enzymes were virtually identical for each of eight strains of Escherichia coli and for two ...

Study of Bacterial Diversity in Poultry Gut Using Denaturing Gradient Gel Electrophoresis

Bacteria play an important role in the initiation and progression of diseases (not only in disease but affect health status of individual). Populations of digestive microflora in chicken gut change with age and also affected by diet, stressors, and performance enhancers as well as in disease conditions. The aim of the pre-sent study was to know bacterial makeup in chicken gut, using denaturing ...

Resolution of Bacterial Proteins by Polyacrylamide Gel Electrophoresis on Slabs

The application of a method of electrophoretic protein analysis to the investigation of the total protein composition of Salmonella typhimurium is described. It involves electrophoresis of many samples simultaneously on a thin slab of acrylamide gel, in the presence of sodium dodecyl sulfate, with the discontinuous buffer system of Laemmli ((19’70) Nature 227, 680485). Up to about 25 samples ca...

Differential Collagen Biosynthesis by Human Neuroblastoma Cell Variants1

Two morphologically distinct types of cells have been observed in cultures of human neuroblastoma cells. One, designated N-type, is com posed of small neuroblast-like cells; the other, designated S-type, is composed of large, substrate-adherent cells. To obtain additional infor mation on the nature of these two phenotypes, we have investigated the collagen biosynthesis in several clones of huma...